MerryCRISPR – CRISPR library generation

Version 2.2: July, 21 2019

• Major overhaul of off-target scoring

  • Replaced for-loops with Pandas apply

  • ~40-fold speed-up

Version 2.1: July, 5 2019

• Introduced new-species subcommand

  • Able to fetch annotations and sequences from Ensembl

  • Can build a Bowtie index from fetched sequences

• Added Documentation

Version 2.0: June, 17 2019

• Initial release of major overhaul of MerryCRISPR:

  • Replaced custom GTF/GFF parsing functions with gtfparse

  • Handling of FASTA files by pyfaidx

  • Removed hardcoding of nuclease information to a csv file, so new nucleases can be easily added.

  • Almost total refactoring of code.

    • Replaced use of lists with Pandas

  • Replaced old Rule Set 2 with a version of Azimuth overhauled for Python 3 compatibility